Sergei Afanasiev - CV Upstream Manufacturing Technician (Biomanufacturing Technician)

1 year in Upstream (Biotechnology), Half-year in analytical laboratory

~Associate Degree, Analytical Laboratory Technology (Saint-Petersburg State Institute of Technology) Среднее профессиональное образование "Технология аналитического контроля химических соединенений", Санкт-Петербургский государственный технологический институт (технический университет)

Operated and controlled Cytiva and Sartorius bioreactors (start-up, process monitoring, parameter control). Maintained strict GMP standards and aseptic conditions in a cleanroom environment. Prepared feed solutions, conducted weighing of raw materials, and managed inventory of consumables. Performed autonomous process execution without direct supervisor oversight. Conducted cell counting using a microscope (hemocytometer / automated counters). Calibrated pH meters, conductivity meters, and peristaltic pumps. Accurately recorded all process data in batch records, logbooks, and material tracking cards. Demonstrated ability to handle expensive laboratory/production equipment with care.

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Partial knowledge of downstream purification processes including:

Solution preparation

Affinity chromatography

Cation-exchange chromatography

Anion-exchange chromatography

Multimodal chromatography

Concentration and diafiltration

Preparation and addition of excipient solutions

Packing and unpacking of chromatography columns

Column packing quality testing
Also
Working with burettes and titrators (manual/automated)

Preparation and standardization of solutions

Density measurement (e.g., using pycnometer, densitometer)

Other common tasks typical of an analytical laboratory (such as pH measurement, filtration, dilution, sample preparation, and routine quality control tests)